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Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Capítulos
- 00:05Título
- 00:30Cell Harvesting and Freezing
- 03:09Cryogenic Disruption of Frozen Cells
- 04:50Affinity Capture of Protein Complexes from Cell Extracts
- 06:02Affinity Capture
- 07:41Results: Comparing Some Different Aspects of Affinity Capture Optimization
- 09:38Conclusion
Here we describe protocols to disrupt mammalian cells by solid-state milling at a cryogenic temperature, produce a cell extract from the resulting cell powder, and isolate protein complexes of interest by affinity capture upon antibody-coupled micron-scale paramagnetic beads.
