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从Cryomilled哺乳动物细胞中的蛋白质复合物亲和捕获

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

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从Cryomilled哺乳动物细胞中的蛋白质复合物亲和捕获

14,449 Views

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10:37 min

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December 09, 2016

DOI:

10.3791/54518-v

DOI:

10.3791/54518-v

•

10:37 min

December 09, 2016

•

7 Views

John LaCava^1,2, Hua Jiang¹, Michael P. Rout¹

¹Laboratory of Cellular and Structural Biology,The Rockefeller University, ²Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine

Summary

这里，我们描述协议破坏由固态研磨哺乳动物细胞在低温下，产生从所得细胞粉细胞提取物，并且在抗体偶联微米级的顺磁珠亲和捕获隔离感兴趣的蛋白质复合物。

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