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In Situ Rotulagem de replicação de DNA mitocondrial em Drosophila Adulto Ovários por Edu Coloração
JoVE Journal
Genetics
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JoVE Journal Genetics
In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

In Situ Rotulagem de replicação de DNA mitocondrial em Drosophila Adulto Ovários por Edu Coloração

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8,822 Views

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10:31 min

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October 15, 2016

DOI:

10.3791/54516-v

DOI:
10.3791/54516-v

•

10:31 min
October 15, 2016

•

2 Views
,
1Lab of Molecular Genetics, National Heart, Lung and Blood Institute,National Institutes of Health

Summary

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Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

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Mitochondrial DNA ReplicationDrosophilaAdult OvariesEdU StainingMitochondrial BiogenesisMitochondrial DNA InheritanceIn Situ LabelingDrosophila MediumAphidicolinEdUOvary DissectionOvariolesFluorescent Dye Penetration

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Cite this Article

Chen, Z., Xu, H. In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining. J. Vis. Exp. (116), e54516, doi:10.3791/54516 (2016).

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