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In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
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In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

8,816 Views

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10:31 min

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October 15, 2016

DOI:

10.3791/54516-v

DOI:
10.3791/54516-v

•

10:31 min
October 15, 2016

•

2 Views
,
1Lab of Molecular Genetics, National Heart, Lung and Blood Institute,National Institutes of Health (NIH)

概要

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Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

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Mitochondrial DNA ReplicationDrosophilaAdult OvariesEdU StainingMitochondrial BiogenesisMitochondrial DNA InheritanceIn Situ LabelingDrosophila MediumAphidicolinEdUOvary DissectionOvariolesFluorescent Dye Penetration

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Cite this Article

Chen, Z., Xu, H. In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining. J. Vis. Exp. (116), e54516, doi:10.3791/54516 (2016).

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