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Chapters
- 00:05Title
- 00:41Tissue Collection and Dissection
- 02:21EdU Labeling, Fixation, and Permeabilization
- 04:17EdU Detection
- 05:55Antibody Labeling
- 07:21Mounting and Imaging
- 08:37Results: EdU Staining of Mitochondrial DNA Replication in Drosophila Ovaries
- 10:06Conclusion
Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.
