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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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JoVE Journal 発生生物学
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

DOI:
10.3791/54492-v

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11:19 min

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December 16, 2016

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, , ,
1Laboratory of Genetics,University of Wisconsin, 2Department of Neurobiology,University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program,University of Pittsburgh School of Medicine

章

  • 00:05標題
  • 00:47Selecting Females for Egg Collection via Interrupted Mating
  • 01:31Preparation of Sperm Solution
  • 02:50UV Treatment of Sperm Solution
  • 03:37Manual Extrusion of Mature Eggs
  • 04:56In Vitro Fertilization
  • 05:51Heat Shock Treatment
  • 07:13Selection of Embryos with a One-cycle Cytokinesis Stall
  • 08:48Results: Heat Shock 2 Treatment of Zebrafish Embryos
  • 09:57Conclusion

概要

自動翻訳

自動翻訳

A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.

タグ

Ploidy ManipulationZebrafish EmbryosHeat ShockDiploidization遺伝学Inbred LinesGenetic ManipulationsMatingEgg ExtrusionSperm SolutionUV TreatmentDissectionTestesHank SolutionPipettingUV Lamp

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