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October 05, 2016
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This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.
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Bogliotti, Y. S., Vilarino, M., Ross, P. J. Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes. J. Vis. Exp. (116), e54465, doi:10.3791/54465 (2016).
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