Journal
/
Genética
/
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

8,814 Views

•

08:59 min

•

October 05, 2016

DOI:

10.3791/54465-v

DOI:
10.3791/54465-v

•

08:59 min
October 05, 2016

•

9 Views
, ,
1Department of Animal Science,University of California, Davis

Summary

Automatically generated

This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.

Explore More Videos

Laser-assisted MicroinjectionCytoplasmic MicroinjectionLivestock ZygotesGenome EditingCRISPR Cas9Transgenic AnimalsBorosilicate Glass CapillaryMicropipette PullerMicroforgePipette DiameterPipette Taper

Read Article

Cite this Article

Bogliotti, Y. S., Vilarino, M., Ross, P. J. Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes. J. Vis. Exp. (116), e54465, doi:10.3791/54465 (2016).

Download .ris file

Copy

Share Video

.

Copy