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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
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遗传学
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JoVE 杂志 遗传学
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

8,814 Views

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08:59 min

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October 05, 2016

DOI:

10.3791/54465-v

DOI:
10.3791/54465-v

•

08:59 min
October 05, 2016

•

9 Views
, ,
1Department of Animal Science,University of California, Davis

Summary

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This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.

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Cite this Article

Bogliotti, Y. S., Vilarino, M., Ross, P. J. Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes. J. Vis. Exp. (116), e54465, doi:10.3791/54465 (2016).

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