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Genetische manipulatie van een onconventionele Gist voor duurzame biobrandstoffen en biochemische Production
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Genetics
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JoVE Journal Genetics
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
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Genetische manipulatie van een onconventionele Gist voor duurzame biobrandstoffen en biochemische Production

Nederlands

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13,016 Views

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10:10 min

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September 20, 2016

DOI:

10.3791/54371-v

DOI:
10.3791/54371-v

•

10:10 min
September 20, 2016

•

12 Views
, , , , , ,
1Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, 2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute,National University of Singapore, 3Food Science and Chemical Engineering,Singapore Institute of Technology

Summary

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We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.

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Genetic EngineeringUnconventional YeastRenewable BiofuelBiochemical ProductionYarrowia LipolyticaPO1G StrainGene DeletionHomologous RecombinationTransformation ProtocolLoxP SitesLEU2 Expression CassetteKU70 GeneDisruption Cassette

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Cite this Article

Yu, A., Pratomo, N., Ng, T., Ling, H., Cho, H., Leong, S. S. J., Chang, M. W. Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production. J. Vis. Exp. (115), e54371, doi:10.3791/54371 (2016).

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