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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
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10:10 min
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September 20, 2016
DOI:
1Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, 2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute,National University of Singapore, 3Food Science and Chemical Engineering,Singapore Institute of Technology
Summary
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We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.
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Yu, A., Pratomo, N., Ng, T., Ling, H., Cho, H., Leong, S. S. J., Chang, M. W. Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production. J. Vis. Exp. (115), e54371, doi:10.3791/54371 (2016).
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