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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
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JoVE Journal 遺伝学
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

DOI:
10.3791/54371-v

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10:10 min

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September 20, 2016

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, , , , , ,
1Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, 2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute,National University of Singapore, 3Food Science and Chemical Engineering,Singapore Institute of Technology

章

  • 00:05標題
  • 01:18Construction of the Disruption Cassette for Deleting KU70
  • 03:08Generation of the Y. lipolytica KU70 Deletion Strain
  • 05:45Cre Recombinase-mediated Marker Rescue
  • 07:59Results: Deletion of KU70 Enhances Gene Deletion Efficiency
  • 09:27Conclusion

概要

自動翻訳

自動翻訳

We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.

タグ

Genetic EngineeringUnconventional YeastRenewable BiofuelBiochemical ProductionYarrowia LipolyticaPO1G StrainGene DeletionHomologous RecombinationTransformation ProtocolLoxP SitesLEU2 Expression CassetteKU70 GeneDisruption Cassette

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