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Reliable and High Efficiency Extraction of Kidney Immune Cells
1Division of Nephrology,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital, 2Division of Biomedical Sciences,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital, 3Division of Endocrinology,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital
Chapters
- 00:05Title
- 01:16Kidney Perfusion
- 02:35Kidney Dissection and Homogenization
- 04:54Density Gradient Separation and Antibody Staining
- 07:14Results: Representative T and Macrophage/Dendritic Cell Isolation
- 08:29Conclusion
Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.
