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Reliable and High Efficiency Extraction of Kidney Immune Cells
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09:15 min
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August 19, 2016
DOI:
1Division of Nephrology,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital, 2Division of Biomedical Sciences,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital, 3Division of Endocrinology,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital
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Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.
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Nistala, R., Meuth, A., Smith, C., Annayya, A. Reliable and High Efficiency Extraction of Kidney Immune Cells. J. Vis. Exp. (114), e54368, doi:10.3791/54368 (2016).
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