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Neuroscience
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荧光活化细胞分选(FACS)和新鲜和冷冻大鼠脑组织的Fos表达神经元基因表达分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
Please note that all translations are automatically generated.
Click here for the English version.
荧光活化细胞分选(FACS)和新鲜和冷冻大鼠脑组织的Fos表达神经元基因表达分析
DOI:
10.3791/54358-v
•
08:37 min
•
August 27, 2016
•
F. Javier Rubio*
1
,
Xuan Li*
1
,
Qing-Rong Liu
,
Raffaello Cimbro
,
Bruce T. Hope
1
Behavioral Neuroscience Research Branch, Intramural Research Program
,
NIDA, NIH, DHHS
,
2
Division of Rheumatology, School of Medicine
,
Johns Hopkins University
Chapters
00:05
Title
00:43
Tissue Collection and Dissection
02:17
Cell Dissociation, Fixation and Permeabilization
05:52
Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个荧光活化细胞分选(FACS)协议来研究分子改建的Fos表达来自新鲜和冷冻的脑组织神经元歌舞团。使用冷冻组织可在多个会话许多脑区的FACS隔离,最大限度地利用宝贵的动物主题。
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