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Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
JoVE Journal
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JoVE Journal Nörobilim
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

DOI:
10.3791/54358-v

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08:37 min

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August 27, 2016

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, , , ,
1Behavioral Neuroscience Research Branch, Intramural Research Program,NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine,Johns Hopkins Üniversitesi

Bölümler

  • 00:05Başlık
  • 00:43Tissue Collection and Dissection
  • 02:17Cell Dissociation, Fixation and Permeabilization
  • 05:52Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
  • 07:16Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we present a Fluorescence Activated Cell Sorting (FACS) protocol to study molecular alterations in Fos-expressing neuronal ensembles from both fresh and frozen brain tissue. The use of frozen tissue allows FACS isolation of many brain areas over multiple sessions to maximize the use of valuable animal subjects.

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