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Voorbereiding en Toepassingen van organotypische thymus Slice Culturen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation and Applications of Organotypic Thymic Slice Cultures
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Voorbereiding en Toepassingen van organotypische thymus Slice Culturen

Nederlands

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7,806 Views

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10:10 min

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August 06, 2016

DOI:

10.3791/54355-v

DOI:
10.3791/54355-v

•

10:10 min
August 06, 2016

•

2 Views
, ,
1Centre de Recherche,Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology,Université de Montréal, 3Department of Medicine,Université de Montréal

Summary

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We beschrijven de bereiding van thymus segmenten die in combinatie met flowcytometrie, kan worden gebruikt om positieve en negatieve selectie van T-cellen ontwikkelen modelleren. Thymus segmenten kunnen ook worden aangepast voor de in situ analyse van thymocyten migratie, lokalisatie en signalering via immunofluorescentie en twee-foton microscopie.

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Cite this Article

Sood, A., Dong, M., Melichar, H. J. Preparation and Applications of Organotypic Thymic Slice Cultures. J. Vis. Exp. (114), e54355, doi:10.3791/54355 (2016).

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