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Preparation and Applications of Organotypic Thymic Slice Cultures
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Preparation and Applications of Organotypic Thymic Slice Cultures

Preparation and Applications of Organotypic Thymic Slice Cultures

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10:10 min

August 06, 2016

DOI:

10:10 min
August 06, 2016

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Summary

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We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.

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