We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.
Sood, A., Dong, M., Melichar, H. J. Preparation and Applications of Organotypic Thymic Slice Cultures. J. Vis. Exp. (114), e54355, doi:10.3791/54355 (2016).