Journal
/
Developmental Biology
/
Imagiologia Cleared embrionário e pós-natal Corações na resolução de célula única
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imagiologia Cleared embrionário e pós-natal Corações na resolução de célula única

português

Automatically Generated

8,024 Views

•

07:30 min

•

October 07, 2016

DOI:

10.3791/54303-v

DOI:
10.3791/54303-v

•

07:30 min
October 07, 2016

•

2 Views
, , , , , , , ,
1Department of Molecular and Cellular Physiology,Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Summary

Automatically generated

We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

Explore More Videos

Single-cell ResolutionCardiac DevelopmentFluorescent ProteinPECAMAlexa Fluor 647DAPICUBICSCALEEmbryonic HeartPostnatal HeartGene FunctionProtein FunctionImagingConfocal Microscopy

Read Article

Cite this Article

Shaikh Qureshi, W. M., Miao, L., Shieh, D., Li, J., Lu, Y., Hu, S., Barroso, M., Mazurkiewicz, J., Wu, M. Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution. J. Vis. Exp. (116), e54303, doi:10.3791/54303 (2016).

Download .ris file

Copy

Share Video

.

Copy