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Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

8,024 Views

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07:30 min

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October 07, 2016

DOI:

10.3791/54303-v

DOI:
10.3791/54303-v

•

07:30 min
October 07, 2016

•

2 Views
, , , , , , , ,
1Department of Molecular and Cellular Physiology,Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Özet

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We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

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Single-cell ResolutionCardiac DevelopmentFluorescent ProteinPECAMAlexa Fluor 647DAPICUBICSCALEEmbryonic HeartPostnatal HeartGene FunctionProtein FunctionImagingConfocal Microscopy

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Cite this Article

Shaikh Qureshi, W. M., Miao, L., Shieh, D., Li, J., Lu, Y., Hu, S., Barroso, M., Mazurkiewicz, J., Wu, M. Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution. J. Vis. Exp. (116), e54303, doi:10.3791/54303 (2016).

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