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イメージングは​​、単一細胞の解像度で胚および出生後の心をクリア
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

イメージングは​​、単一細胞の解像度で胚および出生後の心をクリア

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07:30 min

October 07, 2016

DOI:

07:30 min
October 07, 2016

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Summary

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We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

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