/
/
Caracterización de las células dendríticas derivadas de monocitos humanos mediante imágenes de Citometría de Flujo: una comparación entre dos protocolos de aislamiento de monocitos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,246 Views
•
08:31 min
•
October 18, 2016
DOI:
Summary
Automatically generated
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
Read Article
Cite this Article
Figueroa, G., Parira, T., Laverde, A., Casteleiro, G., El-Mabhouh, A., Nair, M., Agudelo, M. Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols. J. Vis. Exp. (116), e54296, doi:10.3791/54296 (2016).
Copy