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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.' 'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
'Bölümler'
- 00:05'Başlık'
- 01:17Standard Density Gradient Peripheral Blood Mononuclear Cells (PBMC) Isolation
- 02:24Monocyte Purification
- 04:38Cell Surface Staining Analysis by Imaging Flow Cytometry
- 05:46Results: Representative Monocyte Yield and Isolation Analyses
- 07:22Conclusion
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
