This content is Free Access.
9,492 Views
•
10:52 min
•
August 13, 2016
DOI:
Summary
Automatically generated
The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
Read Article
Cite this Article
Vleugel, M., Roth, S., Groenendijk, C. F., Dogterom, M. Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets. J. Vis. Exp. (114), e54278, doi:10.3791/54278 (2016).
Copy