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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Bölümler
- 00:05Başlık
- 00:47Microfluidic Chips
- 02:37Microfluidic Setup: Lipid Preparation and Making PDMS Items
- 04:38Microfluidic Setup: Emulsion-droplet Formation
- 06:15Reconstituting Basic Microtubule Asters
- 07:27Imaging
- 08:48Results: Microtubule Aster Visualization
- 10:07Conclusion
The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
