Rivista
/
/
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

9,494 Views

10:52 min

August 13, 2016

DOI:

10:52 min
August 13, 2016

1 Views
, , ,

Summary

Automatically generated

The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.

Read Article