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Combinando a microscopia Intravital fluorescente (IVFM) com modelos genéticos para estudar a dinâmica de enxertia das células hematopoiéticas para nichos de medula óssea
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Developmental Biology
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JoVE Journal Developmental Biology
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Combinando a microscopia Intravital fluorescente (IVFM) com modelos genéticos para estudar a dinâmica de enxertia das células hematopoiéticas para nichos de medula óssea

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11:06 min

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March 21, 2017

DOI:

10.3791/54253-v

DOI:
10.3791/54253-v

•

11:06 min
March 21, 2017

•

2 Views
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1Wells Center for Pediatric Research, Department of Pediatrics,Indiana University School of Medicine, 2Indiana Center for Biological Microscopy, Department of Medicine,Indiana University School of Medicine, 3Department of Pediatrics,Indiana University School of Medicine

Summary

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Microscopia de fluorescência intravital (IVFM) da calvária é aplicada em combinação com modelos de animais geneticamente para estudar o homing e enxertia das células hematopoiéticas em nichos de medula óssea (BM).

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Wang, L., Kamocka, M. M., Zollman, A., Carlesso, N. Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches. J. Vis. Exp. (121), e54253, doi:10.3791/54253 (2017).

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