Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Lin Wang; Malgorzata M. Kamocka; Amy Zollman; Nadia Carlesso

doi:10.3791/54253

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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

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発生生物学

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JoVE Journal 発生生物学

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

7,806 Views

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11:06 min

•

March 21, 2017

DOI:

10.3791/54253-v

DOI:

10.3791/54253-v

•

11:06 min

March 21, 2017

•

2 Views

Lin Wang*¹, Malgorzata M. Kamocka*², Amy Zollman³, Nadia Carlesso³

¹Wells Center for Pediatric Research, Department of Pediatrics,Indiana University School of Medicine, ²Indiana Center for Biological Microscopy, Department of Medicine,Indiana University School of Medicine, ³小児科,Indiana University School of Medicine

概要

Intravital fluorescence microscopy (IVFM) of the calvarium is applied in combination with genetic animal models to study the homing and engraftment of hematopoietic cells into bone marrow (BM) niches.

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Intravital Fluorescent Microscopy IVFM Hematopoietic Cell Engraftment Bone Marrow Niches Hematopoietic Cell Regeneration Cell-to-cell Interactions Myeloproliferative Disease Notch Signaling EGFP Mouse Bone Marrow Transplant Cell Isolation Cell Suspension Tail Vein Injection

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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

概要

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