Journal
/
Biología del desarrollo
/
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

DOI:
10.3791/54253-v

•

11:06 min

•

March 21, 2017

•

, , ,
1Wells Center for Pediatric Research, Department of Pediatrics,Indiana University School of Medicine, 2Indiana Center for Biological Microscopy, Department of Medicine,Indiana University School of Medicine, 3Department of Pediatrics,Indiana University School of Medicine

Capítulos

  • 00:05Título
  • 01:26Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
  • 03:16Lys-GFP BMT and Intravital Imaging Preparation
  • 05:23In Vivo High Resolution Mouse Calvarium Imaging
  • 07:15Quantitative Analysis
  • 08:14Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
  • 10:10Conclusion

Summary

Traducción Automática

Traducción Automática

Intravital fluorescence microscopy (IVFM) of the calvarium is applied in combination with genetic animal models to study the homing and engraftment of hematopoietic cells into bone marrow (BM) niches.

Tags

Keywords: Intravital Fluorescent MicroscopyIVFMHematopoietic Cell EngraftmentBone Marrow NichesHematopoietic Cell RegenerationCell-to-cell InteractionsMyeloproliferative DiseaseNotch SignalingEGFP MouseBone Marrow TransplantCell IsolationCell SuspensionTail Vein Injection

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/54253?article_type=v

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
check_url/es/54253?article_type=v

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
check_url/es/54253?article_type=v

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
check_url/es/54253?article_type=v

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
check_url/es/54253?article_type=v

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
check_url/es/54253?article_type=v

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
check_url/es/54253?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
check_url/es/54253?article_type=v

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue
check_url/es/54253?article_type=v

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
check_url/es/54253?article_type=v

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Read Article