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DNA-RNA simultânea de extração de sedimentos costeiros e quantificação de 16S rRNA genes e transcritos por PCR em tempo real
JoVE Journal
Environment
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JoVE Journal Environment
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

DNA-RNA simultânea de extração de sedimentos costeiros e quantificação de 16S rRNA genes e transcritos por PCR em tempo real

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17,198 Views

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11:37 min

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June 11, 2016

DOI:

10.3791/54067-v

DOI:
10.3791/54067-v

•

11:37 min
June 11, 2016

•

13 Views
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1Microbiology, School of Natural Sciences,National University of Ireland Galway, 2School of Biological Sciences,University of Essex

Summary

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A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

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DNA-RNA ExtractionCoastal Sediments16S RRNA Genes16S RRNA TranscriptsQuantitative PCRMolecular AnalysisMicrobial AbundanceMicrobial Diversity

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Tatti, E., McKew, B. A., Whitby, C., Smith, C. J. Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR. J. Vis. Exp. (112), e54067, doi:10.3791/54067 (2016).

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