Journal
/
Environment
/
ARN-ADN Extraction simultanée de sédiments côtiers et Quantification de 16S ARNr et Transcriptions par PCR en temps réel
JoVE Journal
Environment
This content is Free Access.
JoVE Journal Environment
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Please note that all translations are automatically generated. Click here for the English version.

ARN-ADN Extraction simultanée de sédiments côtiers et Quantification de 16S ARNr et Transcriptions par PCR en temps réel

français

Automatically Generated

17,188 Views

•

11:37 min

•

June 11, 2016

DOI:

10.3791/54067-v

DOI:
10.3791/54067-v

•

11:37 min
June 11, 2016

•

13 Views
, , ,
1Microbiology, School of Natural Sciences,National University of Ireland Galway, 2School of Biological Sciences,University of Essex

Summary

Automatically generated

A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

Explore More Videos

DNA-RNA ExtractionCoastal Sediments16S RRNA Genes16S RRNA TranscriptsQuantitative PCRMolecular AnalysisMicrobial AbundanceMicrobial Diversity

Read Article

Cite this Article

Tatti, E., McKew, B. A., Whitby, C., Smith, C. J. Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR. J. Vis. Exp. (112), e54067, doi:10.3791/54067 (2016).

Download .ris file

Copy

Share Video

.

Copy