This content is Free Access.
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
17,188 Views
•
11:37 min
•
June 11, 2016
DOI:
Summary
Automatically generated
A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.
Read Article
Cite this Article
Tatti, E., McKew, B. A., Whitby, C., Smith, C. J. Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR. J. Vis. Exp. (112), e54067, doi:10.3791/54067 (2016).
Copy