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In Vitro Colony saggi per Caratterizzazione Tri-potenti cellule progenitrici isolato dal adulti murini Pancreas
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
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In Vitro Colony saggi per Caratterizzazione Tri-potenti cellule progenitrici isolato dal adulti murini Pancreas

italiano

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9,301 Views

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09:31 min

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June 10, 2016

DOI:

10.3791/54016-v

DOI:
10.3791/54016-v

•

09:31 min
June 10, 2016

•

1 Views
, , , , , , , ,
1Diabetes and Metabolism Research Institute,Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Summary

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Nei saggi di colonie in vitro per rilevare auto-rinnovamento e la differenziazione delle cellule progenitrici isolate dal pancreas di topo adulti sono messo a punto. In questi saggi, progenitori pancreatici danno origine a colonie di cellule nello spazio 3-dimensionale in terreno semisolido contenente metilcellulosa. I protocolli per la gestione di singole cellule e caratterizzazione delle singole colonie sono descritti.

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Tremblay, J. R., LeBon, J. M., Luo, A., Quijano, J. C., Wedeken, L., Jou, K., Riggs, A. D., Tirrell, D. A., Ku, H. T. In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas. J. Vis. Exp. (112), e54016, doi:10.3791/54016 (2016).

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