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In Vitro Kolonie assays voor het karakteriseren van Tri-potente Progenitor Cellen Geïsoleerd van de volwassen muizen alvleesklier
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
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In Vitro Kolonie assays voor het karakteriseren van Tri-potente Progenitor Cellen Geïsoleerd van de volwassen muizen alvleesklier

Nederlands

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9,301 Views

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09:31 min

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June 10, 2016

DOI:

10.3791/54016-v

DOI:
10.3791/54016-v

•

09:31 min
June 10, 2016

•

1 Views
, , , , , , , ,
1Diabetes and Metabolism Research Institute,Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Summary

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In vitro testen van kolonies op zelfvernieuwing en differentiatie van progenitorcellen geïsoleerd uit volwassen muizen pancreas gedetecteerd worden gepland. In deze testen pancreatische voorlopercellen leiden tot cel kolonies in 3-dimensionale ruimte-methylcellulose bevattende halfvaste medium. Protocollen voor het manipuleren van enkelvoudige cellen en karakterisering van individuele kolonies worden beschreven.

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Cite this Article

Tremblay, J. R., LeBon, J. M., Luo, A., Quijano, J. C., Wedeken, L., Jou, K., Riggs, A. D., Tirrell, D. A., Ku, H. T. In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas. J. Vis. Exp. (112), e54016, doi:10.3791/54016 (2016).

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