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In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
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Biologie du développement
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Journal JoVE Biologie du développement
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

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09:31 min

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June 10, 2016

DOI:

10.3791/54016-v

DOI:
10.3791/54016-v

•

09:31 min
June 10, 2016

•

1 Views
, , , , , , , ,
1Diabetes and Metabolism Research Institute,Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Summary

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In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.

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In Vitro Colony AssayTri-potent Progenitor CellsAdult Murine PancreasPancreatic Progenitor CellsSelf-renewalDifferentiationHematopoietic Stem CellsPancreatic Cell PopulationsAbdominal CavitySpleenPBSBSADNase ICollagenase BCell IsolationCell Culture

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Tremblay, J. R., LeBon, J. M., Luo, A., Quijano, J. C., Wedeken, L., Jou, K., Riggs, A. D., Tirrell, D. A., Ku, H. T. In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas. J. Vis. Exp. (112), e54016, doi:10.3791/54016 (2016).

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