Identification des conditions critiques pour Immunocoloration dans le puceron du pois embryons: L'augmentation de la perméabilité des tissus et la diminution de la coloration de fond

A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.