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Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

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09:44 min

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February 02, 2016

DOI:

10.3791/53883-v

DOI:
10.3791/53883-v

•

09:44 min
February 02, 2016

•

1 Views
,
1Department of Entomology,National Taiwan University, 2Institute of Biotechnology,National Taiwan University, 3Research Center for Developmental Biology and Regenerative Medicine,National Taiwan University, 4Genome and Systems Biology Degree Program,National Taiwan University and Academia Sinica

Summary

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A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.

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Lin, G., Chang, C. Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining. J. Vis. Exp. (108), e53883, doi:10.3791/53883 (2016).

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