Journal
/
发育生物学
/
Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

9,818 Views

•

09:44 min

•

February 02, 2016

DOI:

10.3791/53883-v

DOI:
10.3791/53883-v

•

09:44 min
February 02, 2016

•

1 Views
,
1Department of Entomology,National Taiwan University, 2Institute of Biotechnology,National Taiwan University, 3Research Center for Developmental Biology and Regenerative Medicine,National Taiwan University, 4Genome and Systems Biology Degree Program,National Taiwan University and Academia Sinica

Summary

Automatically generated

A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.

Explore More Videos

ImmunostainingPea Aphid EmbryosBackground StainingGerm CellsLaboratory StrainHost PlantsParaformaldehydeOvariesPBST

Read Article

Cite this Article

Lin, G., Chang, C. Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining. J. Vis. Exp. (108), e53883, doi:10.3791/53883 (2016).

Download .ris file

Copy

Share Video

.

Copy