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Insan pluripotent kök hücreleri gelen melanosit besleyici içermeyen türetilmesi
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
DOI:

12:21 min

March 03, 2016

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Chapters

  • 00:05Title
  • 00:42Coating of Culture Dishes
  • 01:50Maintenance of Human Pluripotent Stem Cells
  • 03:22Plating hPSCs for Differentiation
  • 05:27Induction of Neuronal Differentiation
  • 07:49Replating in Droplets for Neural Crest Specification
  • 09:31Expanding Melanocyte Progenitors
  • 10:21Results: Derivation of Mature Melanocytes from hPSCs Using a Feeder-free Method
  • 11:47Conclusion

Summary

Automatic Translation

Bu çalışma, bir nöral ile insan pluripotent kök hücreleri pigmentli olgun melanosit üretilmesi ve besleyici içermeyen, 25 günlük protokolünü kullanarak ara madde aşama melanoblast için bir in vitro farklılaşma protokolü tarif eder.

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