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Journal
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Developmental Biology
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从人多能干细胞的黑素细胞的无饲养推导
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从人多能干细胞的黑素细胞的无饲养推导
DOI:
10.3791/53806-v
•
12:21 min
•
March 03, 2016
•
Scott J. Callahan
2
,
Yvonne Mica
,
Lorenz Studer
1
The Center for Stem Cell Biology, Developmental Biology Program
,
Memorial Sloan-Kettering Cancer Center
,
2
Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School
,
Sloan-Kettering Institute for Cancer Research
,
3
Thermo Fisher Scientific
Chapters
00:05
Title
00:42
Coating of Culture Dishes
01:50
Maintenance of Human Pluripotent Stem Cells
03:22
Plating hPSCs for Differentiation
05:27
Induction of Neuronal Differentiation
07:49
Replating in Droplets for Neural Crest Specification
09:31
Expanding Melanocyte Progenitors
10:21
Results: Derivation of Mature Melanocytes from hPSCs Using a Feeder-free Method
11:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个工作描述了一种
在体外
分化协议经由神经嵴,以产生自人多能干细胞色素,成熟的黑素细胞,并使用一个无饲养25日实验方案黑素细胞的中间阶段。
Tags
Keywords: Melanocyte
Pluripotent Stem Cells
Pigmentation
Feeder-free
Differentiation
Drug Discovery
Disease Modeling
Culture Conditions
Laminin
Fibronectin
Mouse Embryonic Fibroblasts
HESC Medium
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