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Feeder-fri Udledning af melanocytter fra menneskelige pluripotente stamceller
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Developmental Biology
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JoVE Journal Developmental Biology
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

Feeder-fri Udledning af melanocytter fra menneskelige pluripotente stamceller

DOI:
10.3791/53806-v

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12:21 min

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March 03, 2016

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, ,
1The Center for Stem Cell Biology, Developmental Biology Program,Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School,Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

Chapters

  • 00:05Title
  • 00:42Coating of Culture Dishes
  • 01:50Maintenance of Human Pluripotent Stem Cells
  • 03:22Plating hPSCs for Differentiation
  • 05:27Induction of Neuronal Differentiation
  • 07:49Replating in Droplets for Neural Crest Specification
  • 09:31Expanding Melanocyte Progenitors
  • 10:21Results: Derivation of Mature Melanocytes from hPSCs Using a Feeder-free Method
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

Dette arbejde beskriver en in vitro differentiering protokol til at producere pigmenterede, modne melanocytter fra humane pluripotente stamceller via en neural crest og melanoblast mellemtrin ved hjælp af en feeder-fri, 25 dage protokol.

Tags

MelanocytePluripotent Stem CellsPigmentationFeeder-freeDifferentiationDrug DiscoveryDisease ModelingCulture ConditionsLamininFibronectinMouse Embryonic FibroblastsHESC Medium

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