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Chapters
- 00:05Title
- 01:02Low Density Embryonic Neuronal Culture
- 03:55Fluorescent Labeling and Immunocytochemistry
- 05:22F-actin Puncta Counting
- 07:34Results: Representative F-actin Synaptic Structures of Rat Cortical Neurons
- 09:21Conclusion
Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.
Tags
QuantificationFilamentous ActinF-actinPunctaRat Cortical NeuronsSynaptic StructuresNeuroscienceSpinogenesisSynaptic PlasticitySynaptic StabilityTechniquePrimary Neuronal CultureIdentificationQuantificationNeuron DamageDrugs Of AbuseCocaineHIV Toxic ProteinsTatJP 120Glass Bottom DishesPoly-L Lysine Working SolutionPlating MediumBrain Harvesting
