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Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
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Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
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February 10, 2016
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Summary
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Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.
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Li, H., Aksenova, M., Bertrand, S. J., Mactutus, C. F., Booze, R. Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons. J. Vis. Exp. (108), e53697, doi:10.3791/53697 (2016).
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