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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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February 22, 2016
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This protocol presents a method to perform quantitative, single-cell in situ analysis of protein expression to study lineage specification in mouse preimplantation embryos. The procedures necessary for collection of blastocysts, whole-mount immunofluorescent detection of proteins, imaging of samples on a confocal microscope, and nuclear segmentation and image analysis are described.
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Saiz, N., Kang, M., Schrode, N., Lou, X., Hadjantonakis, A. Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos. J. Vis. Exp. (108), e53654, doi:10.3791/53654 (2016).
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