Journal
/
Developmental Biology
/
Het bijhouden van cellen in GFP-transgene zebravis Met behulp van de photoconvertible PSmOrange System
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
Please note that all translations are automatically generated. Click here for the English version.

Het bijhouden van cellen in GFP-transgene zebravis Met behulp van de photoconvertible PSmOrange System

Nederlands

Automatically Generated

8,456 Views

•

07:59 min

•

February 05, 2016

DOI:

10.3791/53604-v

DOI:
10.3791/53604-v

•

07:59 min
February 05, 2016

•

5 Views
, , ,
1Medical Faculty Mannheim, Department of Cell and Molecular Biology,Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg,Heidelberg University, 3Excellenzcluster CellNetworks,University of Heidelberg

Summary

Automatically generated

We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

Explore More Videos

PSmOrangePhotoconversionGFPZebrafishCell TrackingConfocal MicroscopyDevelopmental BiologyRegenerationTumor ProgressionInvasion488 Nm Laser561 Nm LaserZ-stackRegion Of InterestPhotoactivation BleachingAcquisition StimulationPhotoconversion Protocol

Read Article

Cite this Article

Beretta, C. A., Dross, N., Engel, U., Carl, M. Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System. J. Vis. Exp. (108), e53604, doi:10.3791/53604 (2016).

Download .ris file

Copy

Share Video

.

Copy