Journal
/
Developmental Biology
/
إنشاء الجينوم تحريره المحفزة الإنسان خطوط الخلايا الجذعية: من استهداف لعزل
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
Please note that all translations are automatically generated. Click here for the English version.

إنشاء الجينوم تحريره المحفزة الإنسان خطوط الخلايا الجذعية: من استهداف لعزل

العربية

Automatically Generated

13,295 Views

•

09:51 min

•

February 02, 2016

DOI:

10.3791/53583-v

DOI:
10.3791/53583-v

•

09:51 min
February 02, 2016

•

13 Views
, ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Summary

Automatically generated

Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

Explore More Videos

Genome EditingHuman Pluripotent Stem CellsDisease ModelingColony IsolationTargetingTransfectionCRISPRZinc Finger NucleaseTALONFeeder CellsTrypsinizationCell Culture

Read Article

Cite this Article

Blair, J. D., Bateup, H. S., Hockemeyer, D. F. Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation. J. Vis. Exp. (108), e53583, doi:10.3791/53583 (2016).

Download .ris file

Copy

Share Video

.

Copy