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Developmental Biology

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JoVE Journal Developmental Biology
Establishment of Genome-edited Human Pluripotent Stem Cell Lines
 

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Article DOI: 10.3791/53583-v • 09:51 min • February 2nd, 2016
February 2nd, 2016 •
1, 1, 1
1Department of Molecular and Cell Biology, University of California, Berkeley

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Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

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Keywords: Genome Editing Human Pluripotent Stem Cells Disease Modeling Colony Isolation Targeting Transfection CRISPR Zinc Finger Nuclease TALON Feeder Cells Trypsinization Cell Culture
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