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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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February 02, 2016
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Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.
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Blair, J. D., Bateup, H. S., Hockemeyer, D. F. Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation. J. Vis. Exp. (108), e53583, doi:10.3791/53583 (2016).
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