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Generazione di primaria fibroblasti culture da orecchio e coda tessuti di topo
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Developmental Biology
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JoVE Journal Developmental Biology
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
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Generazione di primaria fibroblasti culture da orecchio e coda tessuti di topo

italiano

Automatically Generated

32,644 Views

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06:35 min

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January 10, 2016

DOI:

10.3791/53565-v

DOI:
10.3791/53565-v

•

06:35 min
January 10, 2016

•

50 Views
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1Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Summary

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Descriviamo una procedura sperimentale semplice e rapido per la generazione di fibroblasti primari dalle orecchie e le code di topi. La procedura non richiede addestramento speciale animali e può essere utilizzato per la generazione di colture di fibroblasti da spighe conservati a temperatura ambiente per 10 giorni.

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Cite this Article

Khan, M., Gasser, S. Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues. J. Vis. Exp. (107), e53565, doi:10.3791/53565 (2016).

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