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Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
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Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

32,546 Views

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06:35 min

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January 10, 2016

DOI:

10.3791/53565-v

DOI:
10.3791/53565-v

•

06:35 min
January 10, 2016

•

50 Views
,
1Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Özet

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We describe a simple and quick experimental procedure for generating primary fibroblasts from the ears and tails of mice. The procedure does not require special animal training and can be used for the generation of fibroblast cultures from ears stored at RT for up to 10 days.

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Cite this Article

Khan, M., Gasser, S. Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues. J. Vis. Exp. (107), e53565, doi:10.3791/53565 (2016).

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