Dergi
/
Gelişim Biyolojisi
/
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

32,644 Views

•

06:35 min

•

January 10, 2016

DOI:

10.3791/53565-v

DOI:
10.3791/53565-v

•

06:35 min
January 10, 2016

•

50 Views
,
1Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Özet

Automatically generated

We describe a simple and quick experimental procedure for generating primary fibroblasts from the ears and tails of mice. The procedure does not require special animal training and can be used for the generation of fibroblast cultures from ears stored at RT for up to 10 days.

Explore More Videos

Primary FibroblastMouse EarMouse TailCell IsolationCollagenase DigestionCell StrainerCell SuspensionCell Washing

Read Article

Cite this Article

Khan, M., Gasser, S. Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues. J. Vis. Exp. (107), e53565, doi:10.3791/53565 (2016).

Download .ris file

Copy

Share Video

.

Copy